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Accession IconGSE103615

Genome-wide profiling of genes during differentiation of wild type (WT) murine embryonic stem cells (ESCs), scrambled control (SCR) ESCs and Mageb16-depleted (KD) ESCs

Organism Icon Mus musculus
Sample Icon 54 Downloadable Samples
Technology Badge Icon Affymetrix Mouse Genome 430 2.0 Array (mouse4302)

Submitter Supplied Information

Description
The Melanoma-associated Antigen gene family (MAGE) generally encodes for tumour antigens. We recently have identified one of the MAGE gene members, Mageb16 to be highly expressed in undifferentiated murine embryonic stem cells (mESCs). The role of Mageb16 for the differentiation of the pluripotent stem cells is completely unknown. Here we demonstrate that Mageb16 (41 kDa) is distributed in cytosol and/or in surface membrane in undifferentiated mESCs. A transcriptome study was performed with differentiated short hairpin RNA (shRNA)-mediated Mageb16 knockdown (KD ESCs) and scrambled control (SCR) ESCs until a period of 22 days. Mageb16 KD ESCs mainly differentiated towards mesodermal derivatives such as cardiovascular lineages. Mesoderm-oriented differentiation initiated biological processes such as adipogenesis, osteogenesis, limb morphogenesis and spermatogenesis were significantly enriched in the differentiated Mageb16 KD ESCs. Cardiomyogenesis in differentiated KD mESCs was stronger when compared to differentiated SCR and wild mESCs. The expression of non-coding RNA (ncRNA) Lin28a and other epigenetic regulatory genes, nucleocytoplasmic trafficking and genes participating in spermatogenesis have also declined faster in the differentiating Mageb16 KD ESCs. We conclude that Mageb16 plays a crucial role for differentiation of ESCs, specifically to the mesodermal lineages. Regulative epigenetic networks and nucleocytoplasmic modifications induced by Mageb16 may play a role for the critical role of Mageb16 for the ESCs differentiation.
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Total Samples
54
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Samples

Show of 54 Total Samples
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Additional Metadata
1KD d15
male
embryoid bodies (ebs)
1SCR d4
male
embryoid bodies (ebs)
3ESC d12
male
embryoid bodies (ebs)
3KD d4
male
embryoid bodies (ebs)
1KD d12
male
embryoid bodies (ebs)
1ESC d12
male
embryoid bodies (ebs)
2KD d4
male
embryoid bodies (ebs)
3ESC d22
male
embryoid bodies (ebs)
1SCR d15
male
embryoid bodies (ebs)
1ESC d15
male
embryoid bodies (ebs)
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