miR-92 enhances c-Myc induced apoptosis. In the R26MER/MER mouse embryonic fibroblasts (MEFs), a switchable variant of Myc, MycERT2, was knocked into the genomic region downstream of the constitutive Rosa26 promoter, allowing acute activation of c-Myc by 4-OHT-induced nuclear translocation. This in vitro system nicely recapitulates c-Myc-induced apoptosis, as activated MycERT2 induces strong p53-dependent apoptosis in response to serum starvation. Enforced miR-92 expression in three independent R26MER/MER MEF lines significantly enhanced Myc-induced apoptosis.
A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis.
Specimen part
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Dynamic transcriptional events in embryonic stem cells mediated by the super elongation complex (SEC).
Specimen part, Cell line, Treatment
View SamplesMurine ES cell gene expression before RA induction are used to compare gene expression for time-points of 2, 4, 6hrs post-induction.
Dynamic transcriptional events in embryonic stem cells mediated by the super elongation complex (SEC).
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View SamplesThis represents an unbiased evaluation of the transcriptional response in the prefrontal cortex and hippocampus areas in the Df(16)A/+ mice, a mouse model of human 22q11 microdeletion syndrome. These mice were generated by chromosomal engineering and carry a microdeltion of ~1.3Mb in the mouse locus syntenic to the human 22q11.1
Altered brain microRNA biogenesis contributes to phenotypic deficits in a 22q11-deletion mouse model.
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