publication_authors:Kondoh K Results

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Microarray (219)

Platform

Affymetrix Mouse Genome 430 2.0 Array (mouse4302) (224)

Includes Publication

GSE25827

ERK5 Regulates Muscle Cell Fusion through Klf Transcription Factors

Mus musculus
4 Downloadable Samples

Description

In skeletal muscle differentiation, muscle-specific genes are regulated by two groups of transcription factors, the MyoD and MEF2 families, which work together to drive the differentiation process. Here we show that ERK5 regulates muscle cell fusion through Klf transcription factors. The inhibition of ERK5 activity suppresses muscle cell fusion with minimal effects on the expression of MyoD, MEF2, and their target genes. Promoter analysis coupled to microarray assay reveals that Klf-binding motifs are highly enriched in the promoter regions of ERK5-dependent upregulated genes. Remarkably, Klf2 and Klf4 expression are also upregulated during differentiation in an ERK5-dependent manner, and knockdown of Klf2 or Klf4 specifically suppresses muscle cell fusion. Moreover, we show that the Sp1 transcription factor links ERK5 to Klf2/4, and that nephronectin, a Klf transcriptional target, is involved in muscle cell fusion. Therefore, an ERK5/Sp1/Klf module plays a key role in the fusion process during skeletal muscle differentiation.

Publication Title

ERK5 regulates muscle cell fusion through Klf transcription factors.

Sample Metadata Fields

Cell line, Time

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GSE28093

Expression profile of OPC senescence

Mus musculus
6 Downloadable Samples

Description

To identify factors involved in OPC senescence, we compared gene expressions between OPC-CG4, OPC-FCS and OPC-Rev.

Publication Title

Esophageal cancer-related gene 4 is a secreted inducer of cell senescence expressed by aged CNS precursor cells.

Sample Metadata Fields

Specimen part

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GSE17101

Expression profiles of mouse glioma-initiating cell-like subclones

Mus musculus
8 Downloadable Samples

Description

This SuperSeries is composed of the SubSeries listed below.

Publication Title

Sox11 prevents tumorigenesis of glioma-initiating cells by inducing neuronal differentiation.

Sample Metadata Fields

Specimen part, Cell line

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GSE17076

Expression profiling of sox11-expressing glioma-initiating cell-like cells

Mus musculus
4 Downloadable Samples

Description

To identify factors involved in tumorigenicity of glioma-initiating cells (GICs), we compared gene expression in GIC-like cells with and without sox11 expression.

Publication Title

Sox11 prevents tumorigenesis of glioma-initiating cells by inducing neuronal differentiation.

Sample Metadata Fields

Specimen part, Cell line

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GSE17062

Expression profiling of mouse glioma-initiating cell-like cells (GICs) and non-GICs

Mus musculus
4 Downloadable Samples

Description

To identify factors involved in glioma-initiating cells (GICs), we compared gene expression between GIC-like cells and non-GICs.

Publication Title

Sox11 prevents tumorigenesis of glioma-initiating cells by inducing neuronal differentiation.

Sample Metadata Fields

Specimen part

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GSE18052

Analysis of gene expression levels in BBF2H7-/- chondrocytes

Mus musculus
2 Downloadable Samples

Description

BBF2H7 (BBF2 human homolog on chromosome 7), an ER-resident basic leucine zipper transcription factor, is activated in response to ER stress and abundantly expresses in chondrocytes. While BBF2H7 is widely expressed in many tissues and organs, the most intense signals were detected in the proliferating zone of the cartilage. We compared gene expressions in primary cultured chondrocytes prepared from rib cartilage between WT and BBF2H7-/- mice at E18.5. Primary cultured chondrocytes were prepared from E18.5 rib cartilage of WT and BBF2H7-/- mice. Chondrocytes were isolated using 0.2% collagenase D (Roche) after adherent connective tissue was removed by 0.2% trypsin (Sigma) and collagenase pretreatment. Isolated chondrocytes were maintained in -MEM (Gibco) supplemented with 10% FCS and 50 g/mL ascorbic acid. Adenovirus vectors expressing the mouse p60 BBF2H7 (1-377 aa, BBF-N) were constructed with the AdenoX Expression system (Clontech), according to the manufacturers protocol. The cells were infected with adenoviruses 30 h before analysis.

Publication Title

Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis.

Sample Metadata Fields

Specimen part

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GSE21754

Expression data from white adipose tissue of Perilipin A transgenic mice

Mus musculus
2 Downloadable Samples

Description

Perilipin A (PeriA) exclusively locates on adipocyte lipid droplets and is essential for lipid storage and lipolysis. Adipocyte specific overexpression of PeriA caused resistance to diet-induced obesity and resulted in improved insulin sensitivity. In order to better understand the biological basis for this observed phenotype we performed DNA microarray analysis on white adipose tissue (WAT) from PeriA transgenic (Tg) and control wildtype (WT) mice.

Publication Title

Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype.

Sample Metadata Fields

Sex, Specimen part

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GSE45229

Unique pharmacological actions of atypical neuroleptic quetiapine: possible role in cell cycle/fate control

Mus musculus
20 Downloadable Samples

Description

Quetiapine is an atypical neuroleptic with a pharmacological profile distinct from classic neuroleptics. It is currently approved for treating patients with schizophrenia, major depression and bipolar I disorder. However, its cellular effects remain elusive.