Affymetrix microarrays were used to determine the mRNA composition of mRNPs obtained by immunoprecipitation with IRP2 (iron regulatory protein 2).
Identification of target mRNAs of regulatory RNA-binding proteins using mRNP immunopurification and microarrays.
Sex
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ETV1 directs androgen metabolism and confers aggressive prostate cancer in targeted mice and patients.
Specimen part, Cell line, Treatment
View SamplesChromosomal rearrangements involving ETS factors, ERG and ETV1, occur frequently in prostate cancer. We here examine mouse prostate cells from WT mice with s with T-ETV1 mice, which contains express the truncated human ETV1 under the endogenous Tmprss2 promoter. ETV1 expression can be tracked by GFP expression.
ETV1 directs androgen metabolism and confers aggressive prostate cancer in targeted mice and patients.
Specimen part
View SamplesWe used microarrays to detail the global gene expression and identified differentially expressed gene list between wild-type anterior prostates and Ptenpc-/- anterior prostates, Ptenpc-/-Smad4pc-/- and Ptenpc-/- anterior prostates, Ptenpc-/-p53pc-/- and Ptenpc-/- anterior prostates at 15 weeks of age.
SMAD4-dependent barrier constrains prostate cancer growth and metastatic progression.
Age, Specimen part
View SamplesRNAseq analysis of cloche m39 mutant zebrafish embryos and wild type siblings at 90% epiboly - tailbud stage Overall design: In order to isolate the cloche gene, RNAseq was performed on a deletion allele of the zebrafish cloche mutant. RNA was extracted from individual embryos at a stage the cloche gene was predicted to be expressed based on previous literature. RNA from the respective genoptypes was then pooled and subjected to RNAseq analysis.
Cloche is a bHLH-PAS transcription factor that drives haemato-vascular specification.
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