publication_authors:Ordas A Results

Showing 3 of 3 results

Sort by

Hide non-downloadable experiments

Filters

Technology

Microarray (15)

Rna-seq (3)

Platform

Affymetrix Mouse Genome 430 2.0 Array (mouse4302) (15)

Illumina HiSeq 2000 (3)

Includes Publication (3)

SRP018699

Gene expression profiling of zebrafish embryos at 5 days post fertilization [Illumina RNA-Seq]

Danio rerio
3 Downloadable Samples
IlluminaHiSeq2000

Description

We compared Agilent custom made expression microarrays with Illumina deep sequencing for RNA analysis of zebrafish embryos 5 days post fertilization, showing as expected a high degree of correlation of expression of a common set of 15,927 genes for untreated fish. The transcriptomes were also compared for fish injected in the yolk with Mycobacterium marinum Overall design: This RNA deep sequencing study was designed to determine the gene expression profile of zebrafish embryos 5 days post fertilization. We also have compared expression with embryos that were injected with Mycobacterium marinum in the yolk at 2 hours post fertilization. After injections embryos were transferred into fresh egg water and incubated at 28Â°C. 150 embryos of mock-injected embryos or 200 embryos injected with 12 CFU bacteria were snap-frozen in liquid nitrogen, and total RNA was isolated using TRIZOL reagent.